Alteration of chromatin high‐order conformation associated with oxaliplatin resistance acquisition in colorectal cancer cells

Oxaliplatin is a first-line chemotherapy drug widely adopted in colorectal cancer (CRC) treatment. However, large proportion of patients tend to become resistant oxaliplatin, causing fail. At present, researches on oxaliplatin resistance mainly focus the genetic and epigenetic alterations during evolution, while characteristics high-order three-dimensional (3D) conformation genome are yet be explored. In order investigate chromatin alteration resistance, we performed multi-omics study by combining DLO Hi-C, ChIP-seq as well RNA-seq technologies established oxaliplatin-resistant cell line HCT116-OxR, control HCT116. The results indicate that 19.33% regions have A/B compartments transformation after further analysis genes converted reveals acquisition tumor cells related reduction reactive oxygen species enhanced metastatic capacity. Our research spatial structural difference between CRC based Hi-C other omics experiments. More importantly, provide potential targets for treatment new way behavior under perspective 3D alteration.